Purification of Sea Water by Anion Exchange. I. Purification of Sea Water by Use of Weakly Basic Anion Exchange Resins

Purification of DNA by anion-exchange chromatography.

Column chromatography has evolved to provide a rapid and effective alternative to more laborious methods for preparing high-quality DNA, such as CsCl-gradient centrifugation. This unit describes the use of a column made of a unique anion-exchange resin that selectively binds nucleic acids, allowing rapid separation of DNA from contaminating RNA, proteins, carbohydrates, and metabolites. The pro...

Recycling of anion-exchange resins for plasmid DNA purification.

Plasmid DNA of high purity is critical for molecular biology studies such as subcloning, DNA sequencing, vector construction and expression and functional analysis. The classical method of plasmid DNA purification using cesium chloride/ethidium bromide buoyant-density centrifugation is generally time-consuming and expensive and has gradually been replaced by the use of silica-based, anion-excha...

Trace contamination following reuse of anion-exchange DNA purification resins.

Optimization of Dynamic Binding Capacity of Anion Exchange Chromatography Media for Recombinant Erythropoietin Purification

  Background:The dynamic binding capacity (DBC) of a chromatography matrix in protein purification is the amount of the total protein absorbed into the matrix, before occurrence of a significant break in the breakthrough curve. Optimization of the process criteria for maximum DBC avoids extra process scale-up and reduces ...

Extraction of Carbon Dioxide from Sea water by Ion Exchange Resin Part II: Using Strong Base Anion Exchange Resin